Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 97)

Chapter 6. Data Formatting and Reports
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To change curve fit options or axis types, open the Standard Curve Report (see
page 82) and select the desired regression method and axis type from the buttons in
the dialog box.
Reliability of Calculated Concentrations
Results calculated from mean absorbance data outside the range of the standard curve
may be unreliable. (For the purposes of this discussion, the range of the standard
curve is defined as the absorbance range between the highest and lowest mean
absorbances of the standards used to form the standard curve.) Calculated results that
are determined from mean absorbance results outside the range of the standard curve
are extrapolations, and should be treated as such when determining the final results of
your analysis. Microplate Manager indicates in the Unknown Concentration
Report when the standard curves are extrapolated to determine the concentration of
an unknown.
Results calculated from mean absorbance results at the extremes of the range of the
standard curve may also be unreliable. The linear regression, when properly fitted,
generally produces reliable results at the extremes of the range of the standard curve.
However, the quadratic, cubic, and logistic methods, by their mathematical natures,
can produce unreliable results at the extremes of the standard curve. None of the
methods will give a unique concentration for a given absorbance if the calibration
data is not monotonic in x (i.e., there is more than one x value associated with a
unique y value).
Custom Reports
Microplate Manager currently provides a Custom Report template for generating a
Bovine Spongiform Encephalopathy (BSE) Screening Report. In the future,
additional custom report templates will be added.
With the Data File open, select Custom Reports from the VIEW menu. In the Custom
Report Options dialog, select BSE from the list of reports.
Select Exclude Undefined Wells if you want to exclude absorbance data from
undefined wells in the resulting report. When you click on OK, Microsoft
® Excel will
automatically launch and the report will be displayed as an Excel workbook.