Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 81)

Chapter 5. Templates
73
Click on the Enter Manually button to directly input the dilutions of your unknowns.
Then type in the dilution factor in the appropriate fields in the Dilut. column.
The number in the Dilut. column is the dilution factor, not the final concentration.
For example, if the first unknown has a dilution factor of 1 (i.e., no dilution), and
each unknown is twice as diluted as the previous unknown, enter 2 for the second
unknown, 4 for the third unknown, 8 for the fourth unknown, etc.
The software can calculate the dilution series for you. Click on the Enter
Automatically button, then click on the button that identifies the least diluted
unknown and enter that dilution and the dilution factor in the appropriate fields.
Click on Calculate when all the information is entered. The dilution factors in the
Dilut. column will be automatically updated.
To use the automatic calculation feature, the least diluted unknown must be either the
first unknown (U1) or the last.
Repeat this procedure for each defined assay on your plate.
To implement your changes, click on OK.
Using External Standards
If you run the same assay repeatedly, you may not want to include standards on every
plate. Microplate Manager allows you to run your standards once, then include them
in subsequent readings.
To do so, with your template displayed, select Standard Curve Report or Unknown
Concentration Report from the VIEW menu and click on the tab of the desired
assay.
Choose External Standards... from the OPTIONS menu to open the External
Standards dialog.