Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 12)

Microplate Manager User Guide
4
Data Files
Data Files contain the data from a microplate reading. They have the extension
.mpm.
For an Endpoint reading, this data consists of the individual absorbance readings
from the wells on the microplate. For a Kinetic reading, this data is the time course
series of the individual absorbance readings. A Multiple Plate reading creates a new
Data File for each plate; each file contains Endpoint absorbance measurements.
The Data File also contains a plate template, which contains all the information
necessary to analyze the absorbance data, including a description of the assays, well
types, concentrations, dilutions, and the regression method used for computing
unknowns from standards. When the Data File is first created after a reading, the
template stored in the Protocol File is copied into the Data File. You can later edit the
template or completely replace it with a template from another Data File.
Finally, the Data File includes some information on how the plate was read (e.g.,
measurement wavelength, date/time of reading).
New Features in Version 5.2
Version 5.2 of Microplate Manager includes the following new features:
• Support for Bio-Rad's Model 680 microplate reader.
• A Custom Reports option for generating custom reports using Microsoft
® Excel
templates.
• A new Custom Report for Bio-Rad's BSE test kit.
• Three new regression methods for generating standard curves: Five-Parameter
Logistic (Cook), Five-Parameter Logistic (Rodbard), and Point to Point.
• Additional standard curve features.
• An initial wait time option for Kinetic Protocols.