Bio-Rad Model 680 Microplate Reader Accessories User Manual

Page 45

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Chapter 4. Protocols

37

Figure 27. Absorbance Delay dialog box.

In the Absorbance Delay dialog box, click on the Use Absorbance Delay checkbox
to enable this feature. Select the row and column number of the well whose
absorbance you want to monitor from the pull-down lists. Under Acquire when the
reading is
, specify an absorbance that is above or below a certain OD value. Below
will begin data collection when the measured absorbance is equal to or below the
specified OD, and Above will begin data collection when the absorbance is equal to
or greater than the specified OD.

Click on the OK button to implement your changes.

Ratio Delay, available for dual wavelength ratio measurements, delays data
collection until a selected well reaches a specified measurement/reference wavelength
ratio. Click on the Ratio Delay button, and enter a measurement/reference ratio in the
appropriate field.

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