Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 29)

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4. Protocols
Protocols contain the settings for controlling the microplate reader. To read a
microplate, you must first open a Protocol window and specify the settings.
Some of the features and functions in the Protocols are different depending on the
type of microplate reader you are using. These differences are noted in the text. The
figures depict some functions that are only available with certain microplate readers.
Microplate Manager has three general types of Protocols: Endpoint, Kinetic, and
Multiple Plate.
• Endpoint Protocols are used to acquire a single absorbance reading from each
well of a microplate.
• Kinetic Protocols are used to acquire a series of absorbance readings from each
well over a user-defined interval. You can use these Protocols to calculate
reaction velocities.
• Multiple Plate Protocols are used to collect Endpoint data from a series of
plates. The plates can be loaded by a plate loader for full automation, or they can
be loaded by hand. Multiple Plate Protocols save each successive Endpoint data
set in a separate file, and can also export the data in text format.
First you select the type of Protocol you want to use from the FILE menu, then you
specify the settings for your particular reading, and finally you take the reading. If
you want to reuse the particular Protocol you’ve created, you can save it as a
Protocol file.
Reader Identification
When you select a new or existing Protocol from the FILE menu, the software first
scans for the SCSI or serial port connection to the selected microplate reader.