Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 86)

Microplate Manager User Guide
78
Limit, Matrix and Normalization Reports
The Limit Report, Matrix Report, and Normalization Report allow you to set
upper and lower absorbance or velocity limits on your data and display the results.
Setting Data Limits
To set data limits, first open the Limit, Matrix, or Normalization Report from the
VIEW menu, then select Limits... from the OPTIONS menu. This will open the
Limits dialog.
Figure 57. Limits dialog.
Limits are assigned independently for each assay in a Protocol. When there is more
than one assay, tabs labeled with each assay number appear in the dialog box. To
switch from one assay to another, click on the appropriate tab.
For the Limit and Matrix Reports, if Use Default Limits is selected, the unknown well
with the greatest absorbance or adjusted velocity will be used as the upper limit, and
the unknown well with the lowest absorbance or adjusted velocity will be used as the
lower limit. If no unknowns are specified, the default value for both upper and lower
limits is 0.000. (For information about default limits in the Normalization Report, see
page 80.)
To set your own limits, select the Use Custom Limits option and enter numbers in the
appropriate fields.