Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 95)

Chapter 6. Data Formatting and Reports
87
Figure 65. Analysis of Variance box.
In the Analysis of Variance dialog box, the key statistics are listed in the last
column. Within this last column, there are three subcolumns. From left to right, these
subcolumns show the F statistic, the significance, and the probability that the
difference between the two curves is significant. There are four degrees of
significance: not significant, significant, highly significant, and extremely significant.
These are indicated by the following symbols: ns, *, **, ***.
If there is a significant difference between standard curves that should be essentially
identical, the user should investigate the source of this difference.
Note that these statistics depend on the type of curve fit chosen. Changing the
regression method and axis type will change the analysis of variance.
This information will also be printed at the bottom of the Standard Curve Report.
A complete discussion of the analysis of variance is beyond the scope of this manual.
For more information, please refer to Biometry by Robert R. Sokal and F. James
Rohlf, W.H. Freeman and Company, New York, 1981.
Unknown Concentrations Report
The Unknown Concentrations Report shows the calculated concentrations of the
unknown samples and the dilution and concentration information for all standards.
F statistic Significance Probability