Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 76)

Microplate Manager User Guide
68
Replicate Groups
A replicate group is a group of wells with the same standard or unknown. For each
replicate group, a mean absorbance value (in the case of Endpoint or Multiple Plate
Protocols) or velocity (in the case of Kinetic Protocols) is calculated, along with the
standard deviation and coefficient of variation for the replicate group. If you have
defined multiple blank, positive control, or negative control wells in a template, all
wells of each type are treated like a replicate group, and the same statistical analyses
are performed (mean, standard deviation, and coefficient of variation).
Defining Replicate Groups in a Template
You should define any assays before specifying well types.
To define a replicate group on a template, click on the Standard or Unknown button
on the toolbar, then click on the Turn Off Autofill button and drag across a group of
wells. All the wells you select will have the same number, indicating that they are in
the same replicate group.
Figure 50. Defining a replicate groups of unknowns.
Dragging across another group of wells will assign those to a second replicate group,
and so on.
Suppose you have several replicate groups aligned in single rows or columns. If there
are the same number of replicates in each group, you can use the Autofill buttons and
the replicate group number field to define these groups quickly and easily.
Turn Off Autofill
selected