Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 125)

117
8. Quick Guides
Creating a New 96-Well Template
See Chapter 5 for details.
1. Choose New Template > 12 x 8 from the FILE menu.
2. Click on the Define a new assay button on the template toolbar and drag on the
template to define the location of each separate assay.
3. Use the formatting buttons on the template toolbar (Standard, Unknown,
Positive Control, Negative Control, Blank) to define each well in each assay.
Click on a button and drag in the defined assay to format the wells.
4. Set the concentrations of the standards by choosing Edit Standard Conc... from
the EDIT menu.
5. Set the dilutions of the unknowns by choosing Edit Unknown Dilutions... from
the EDIT menu.
6. Choose Assay Info... from the ASSAY menu to customize the name and color of
each assay in the template and enter specific notes about each assay that will be
printed with reports.
7. For each assay, choose the type of regression and the type of axes used to
generate the Standard Curve and Unknown Concentration reports:
a. Select Standard Curve Report from the VIEW menu.
b. Use the tabs to select the assay.
c. Choose the desired regression method from the Regression Type pulldown
list.
d. Choose the desired type of axes from the Transformation pulldown list.
e. Repeat steps b–d for the other assays.
f. Close the Standard Curve Report window.
8. For each assay, select the external standards (if any) to be used: