Read speed (model 680 only), Setting the measurement wavelength – Bio-Rad Model 680 Microplate Reader Accessories User Manual

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Microplate Manager User Guide

26

or Save Protocol As... from the FILE menu, or click on the Save button on the main
toolbar.

Protocols are not automatically saved when you click on Run. To preserve changes to

a Protocol File, you must save it.

When a template is saved in a Protocol File, it will remain there until you select a
different template.

If you create or edit a template in one Protocol and want to copy it to another

Protocol, you must first save it as a separate Data File. To do so, you have to click on
Run. This will create a Data File with a copy of the template in it. You can then save
this Data File and open it from within the new Protocol.

Read Speed (Model 680 only)

The Model 680 microplate reader has the ability to read at two different speeds: Fast
Read and Step Read. Select the option from the pulldown menu.

Figure 16. Reading speed control (Model 680 only).

Setting the Measurement Wavelength

Microplate Manager will read a plate in either a single-wavelength or a dual-
wavelength mode. To select the filter wavelength (in nanometers) for measurement
and reference readings, go to the Reading Parameters section of the Protocol dialog
box.

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