Metrohm 833 IC Liquid Handling Unit - Manual (Page 64)

7 833 IC Liquid Handling Dialysis Unit
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
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7.4 Assembling the dialysis cell
This section describes the initial assembly of the dialysis cell. Before it
is used a dialysis membrane must be inserted. This is done as follows
(see Fig. 15):
1 Prepare dialysis cell
• Remove dialysis cell 42 (6.2729.100) from the packaging and
screw out the four 6.2744.060 Blank stoppers .
• Use the 6.2621.070 Allen key to loosen the 5 screws 47
completely, remove lower part 46 from upper part 43 and
remove sealing ring 44.
2 Clean dialysis cell
• Thoroughly rinse sealing ring 44, upper part 43 and lower
part 46 of the dialysis cell with ultrapure water and dry with N
2
or a lint-free tissue.
Only ultrapure water or ethanol should be used for cleaning the
dialysis cell, other organic solvents (e.g. acetone) will damage the
Plexiglas cell!
3 Prepare dialysis membrane
• Use the 6.2831.010 Tweezers to remove a new dialysis
membrane 45 (e.g. 6.2714.010) from the packaging and
immerse in a Petri dish filled with ultrapure water for approx. 2
min until the membrane is thoroughly soaked with water.
4 Insert dialysis membrane
• Place upper part 43 on a paper tissue with its inner side
uppermost.
• Place sealing ring 44 in the recess provided for it in upper
part 43.
• Use the 6.2831.010 Tweezers to place the wet dialysis
membrane 45 (e.g. 6.2714.010) inside sealing ring 44 on
upper part 43.
Make sure that the dialysis membrane soaked with water does not dry
out before insertion as otherwise it cannot be used !
5 Close dialysis cell
• Place lower part 46 on upper part 43 so that the two parts are
properly aligned with each other.
• Use the 6.2621.070 Allen key to screw in the 5 screws 47
completely and tighten them up well.