Metrohm 833 IC Liquid Handling Unit - Manual (Page 69)

7.5 Making the capillary connections
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
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• Immerse the other end of aspiration tubing 16 in the sample
container and fix it in position. Ultrapure water is normally
used as the acceptor solution; it must first be degassed (with
N
2
, He or vacuum).
3 Connect dialysis cell
• Screw PTFE capillary 60) (6.1803.040; L =1 m) to PEEK
coupling 21 (6.2744.034) on the delivery side of the pump
tubing with the orange-yellow stoppers of channel 1 from
the 833 Dialysis Unit using a PEEK compression fitting 17
(6.2744.010) (see Fig. 7 and Fig. 21). Lead the other end of
the capillary through one of the feedthroughs 14 at the front
or the side of the 820 Separation Center in the column
compartment and screw it on to inlet opening 50 of upper
part 43 of the dialysis cell with a PVDF compression fitting 64
(6.2744.000) (see Fig. 15 and Fig. 21).
• Screw PEEK capillary 61 (6.1831.050; L =40 cm)(see Fig. 21)
to outlet opening 49 of cell upper part 43 of the dialysis cell
using a PVDF compression fitting 64 (6.2744.000) (see Fig.
15).
Use only the above-mentioned 6.2744.000 PVDF compression
fittings for the dialysis cell connections. If 6.2744.010 PEEK
compression fittings are used then stress cracks could occur in the
dialysis cell !
4 Connect injection valve
• Screw the PEEK capillary 61 (6.1831.050; L = 40 cm)
attached to outlet 49 to connection "1" of valve A 56 using a
6.2744.010 PEEK compression fitting.
• Screw PEEK capillary 62 (6.1831.060; L =1 m) to connection
"2" of valve A 56 using a 6.2744.010 PEEK compression
fitting and lead it out of the column compartment through one
of the feedthroughs 14 on the front or side of the 820
Separation Center. Attach the other end of the PTFE capillary
62 to PEEK coupling 18 on the suction side of the orange-
yellow pump tubing of channel 2 from the 833 Dialsis Unit
using a PEEK compression fitting 17 (6.2744.010) (see Fig. 7
and Fig. 21).
5 Connect waste container
• Screw PTFE capillary 63 (6.1803.040; L =1 m) (23 in Fig. 7)
to PEEK coupling 21 (6.2744.034) on the delivery side of the
pump tubing with the orange-yellow stoppers of channel 2
from the 833 Dialysis Unit using a PEEK compression fitting
17 (6.2744.010) (see Fig. 7 and Fig. 21). Lead the other end
of the capillary into a sufficiently large waste container and fix
it in position.