Metrohm 833 IC Liquid Handling Unit - Manual (Page 81)

8.3 Connect filtration cell
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
73
7 Tubing connection injection valve – waste
• Insert 6.2744.020 Coupling (from the 820 accessories) into
connection 13 provided for it in the 820 IC Separation Center.
• Screw PTFE aspiration tubing 63 onto the 6.2744.020
Coupling inserted in connection 13 and lead it into the waste
bottle.
8 Rinse ultrafiltration cell
• Each time that a new filtration membrane is inserted any air
that may still be contained in the filtration cell or tubing must
be removed. This is done by rinsing the cell and all the tubing
with e.g. ultrapure water:
• Immerse aspiration tubing 16 (see Fig. 24) in the rinsing
solution (degassed ultrapure water).
• Switch on the peristaltic pump in the «IC Net» software.
• Rinse the filtration system with ultrapure water for approx. 5
min. Check that the same amount of solution emerges from
both the waste outlets in the waste bottle.
• Check all tubing from the storage containers to the tubing
cassettes and filtration cell and up to the waste bottle for
leaks. If any leaks are found then the corresponding
connection must either be tightened up or replaced.
• If any air bubbles remain in the dialysis cell then screw off
PEEK capillary 80 (filtrate) from outlet 71 and the filtration cell
and wait until the air bubbles have vanished. Then screw the
tubing back onto the filtration cell again.
8 Closing down the filtration cell
• If the filtration cell is not to be used for a long time then it
must be rinsed with ultrapure water for approx. 10 min. The
inlet and outlet tubing is then screwed off from the filtration
cell and openings 71, 73 and 74 (see Fig. 22) are each
closed with a 6.2744.060 Blank stopper.
• If the filtration cell is not to be used for a long time then the
filtration membrane must be removed and the cell cleaned,
see Section 9.2.8.1 and Fig. 22.
With 820.0X20 IC Separation Center equipped with two injection
valves it is possible to fill both sample loops from the same 833 IC
Liquid Handling Ultrafiltration Unit. In this case connection "1" at valve
A (sample loop outlet) must be connected to connection "2" of valve B
(sample loop inlet) using a 6.1831.040 PEEK capillary (15 cm).