Metrohm 833 IC Liquid Handling Unit User Manual

Page 73

Advertising
background image

7.6 Optimizing the dialysis

833 IC Liquid Handling Unit / Instructions for Use 8.833.1003

65

1 Measure standard directly

Immerse the aspiration tubing 16 of the acceptor solution in

the required ionic standard. This should have approximately

the same total ionic concentration as the sample.

Start a determination in «IC Net» to measure the standard

directly; the program could look like that shown below:

Immerse acceptor solution aspiration tubing 16 (see Fig.

21)in the acceptor solution again and rinse the acceptor

channel for approx. 2 min.

Switch pump of the 833 IC Liquid Handling Dialysis Unit off.

2 Dialyze the standard and measure it

Set the dialysis time to 5 min in the program (see

Section 7.3.1/7.3.2).

Immerse sample aspiration tubing 16 in the standard used in

item 1.

Start the determination in IC Net and wait until the

chromatogram has been evaluated.

3 Further measurements

Increase the transfer time in the program (see

Section 7.3.1/7.3.2) in steps of 5 min until the measured

values are constant.

Carry out measurements as described in item 2.

4 Determine the optimal dialysis time

Plot the relationship between the measured peak areas or

concentrations as a function of the dialysis time (i.e. the

recovery rate) and determine the optimal dialysis time.


Not for dialysis without suppression

Advertising