Bio-Rad S3™ Cell Sorter - Manual (Page 100)

Maintenance
90 | S3 and S3e Cell Sorters
7. Place a lint-free cloth in the nozzle chamber under the nozzle tip to prevent dropping the
nozzle into the instrument.
8. Rotate the black collar holding in the nozzle until it comes loose.
Note: There is a small O-ring that will be removed as well.
9. Pull out the small white nozzle tip.
If sonication is desired or necessary:
10. Place the tip into a tube containing DI water.
11. Cap the tube.
12. Place the tube into a sonicator for several minutes.
13. Proceed to step 14.
If sonication of the tip is not performed:
10. Using a 1 ml syringe filled with ethanol, press against the top and eject ethanol through the
nozzle tip, rotating between the 100 µm tip and the larger opposite end to free clog.
11. Watch the resulting stream. The fluidic stream should be coming out straight from the tip.
12. Repeat if necessary until the stream is coming out straight.
13. Proceed to step 14.
14. Place the O-ring at the point on the nozzle where the bottom of the black collar will be.
15. Place the nozzle tip back onto the nozzle assembly by pushing it up using the sides of the
tip. Avoid touching the end.
16. Secure the black retaining collar until finger tight.
17. Remove the lint-free cloth from nozzle chamber.
18. Press the stage back down and tighten the thumb screw.
19. Click the blue forward arrow.
Fig. 100. Nozzle swap wizard window.