Bio-Rad S3™ Cell Sorter - Manual (Page 38)

ProSort Software
28 | S3 and S3e Cell Sorters
Fig. 25. Sample Calibration Warning dialog box.
Fig. 26. Calibration Sample Offset window.
3.3.6 Decontaminate
An administrator can run the decontaminate procedure if the system shows signs of heavy
background, noticeable debris when running water, or contamination. The complete
internal fluidic system will be decontaminated. Follow the wizard procedure located in
Section 10.7, Decontamination.
3.3.7 Calibrate Sample Pressure Offset
If the system shows an event rate of 0 when running calibration beads in the low setting, or if
it shows 500+ events, the offset will need to be reset. Using this tool will reset the differential
offset sample pressure, which will return the preset sample flow rates (low/med/high) back to
their correct ranges.
1. Load calibration beads into the sample station and move loading stage to the run position.
2. Click
Calibrate Sample Pressure Offset.
A warning window will appear asking if the user wants to continue (Figure 25). ProLine
calibration beads must be running for this calibration process.
The system will automatically begin the calibration process (Figure 26). Once complete the
system offset pressure has been calibrated.