Bio-Rad S3™ Cell Sorter - Manual (Page 15)

Instruction Manual | 5
2.2 Fluidics System
2.2 Fluidics System
The S3 and S3e fluidics system consists of the bulk fluidics, loading stage, nozzle, and sort
collection chamber. The fluidics system supplies sheath fluid, DI water, and sample to the
nozzle, and then collects the waste for proper disposal.
CAUTION: Biohazard! Biosafety is of utmost importance while operating this
instrument. Consult with your local safety officer or review local, state, and federal
regulations to ensure proper handling and disposal of biohazardous substances.
2.2.1 Bulk Fluidics
The S3 and S3e system includes two empty containers as part of the bulk fluidics. Table 6
describes the function of each container.
Table 6. Containers and their functions.
Label
Container Function
Biohazardous The waste container holds the system’s fluid after it has run through the nozzle
Waste and waste lines. This container has a red cap and holds 4 liters of fluids.
Approximately 9 hours of run time can be performed until the empty container
is filled. The fluid collected in this container should be decontaminated as
appropriate to the application and samples being run on the instrument. Please
verify proper treatment and disposal with your safety officer or local health and
safety bodies
Sheath Fluid The sheath fluid container holds the 8x sheath fluid for the system. This container
has a white cap and holds 4 liters of fluid. If preferred, 1x sheath fluid may be used
instead. In this case, the DI water container will not be used to dilute the sheath,
but only for rinsing and cleaning. If 1x sheath fluid is used, ensure the option is
checked in the Global Preferences of the software. This setting will apply globally
to the system when set by an administrator. A full container of 8x sheath fluid
has approximately 50 hours of run time, while a full tank of 1x sheath fluid has
approximately 9 hours of run time
Deionized water The DI water container holds deionized water for mixing with the 8x sheath fluid to
(DI water) create a 1x sheath fluid. This container has a blue cap and holds 4 liters of DI
water with a run time of approximately 9 hours between refills when using 8x
sheath fluid. The DI water is also used for cleaning at the end of the day and to
rinse the sample probe between samples
Note: The ProFlow ™ 8x sheath fluid is diluted with the DI water using an internal fluidics chamber.
Each container uses a quick disconnect
system for easy swapping (Figure 4).
Both the DI water and the sheath fluid
containers have one connector, while
the waste container has two connectors
for waste fluid from the sorting chamber
and the washing station. Located above
the containers inside the instrument
is a magnetic holder for each quick
disconnect. The magnetic holder will
keep the quick disconnect away from
exchange area.
Fig. 4. Fluidics container highlighting the quick
disconnect system for the sheath fluid container.
Sheath fluid cap assembly
Sheath fluid container
Quick disconnect