Bio-Rad S3™ Cell Sorter - Manual (Page 127)

Instruction Manual | 117
13
S3
™
and S3e
™
Cell Sorter
Specifications
The specifications for the S3 and S3e Cell Sorters are shown in Table 31.
Table 31. S3 and S3e Cell Sorter specifications.
Performance
Droplet frequency 37–43 kHz
Sort type True jet-in-air, high performance sorting
Sort rate No hardware limitations, limited only by droplet frequency and application
Sorting purity >99% pure
Sensitivity <125 MESF for FITC and PE
Temperature control Sample and collection temperature control from 4–37°C
Optics Lasers Single laser: 488 nm 100 mW
Single laser upgrade: 561 nm 100 mW or 640 nm 100 mW
Dual laser: 488 nm 100 mW and 561 nm 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
4 fluorescent detectors with PMT
Fluidics Nozzle 100 µm fixed
Pressure 30 psi fixed
Sheath Onboard dilution of 8x sheath or direct use of 1x sheath
Automation Auto alignment
Auto drop delay calibration
Auto droplet monitoring
Auto stream to laser alignment
Sorting Directions Two way
Modes Single, purity, enrich
Collection Up to 5 x 5 ml or 1.5 ml sample tube each direction
Microscope slides
2 x 8 micro well strip each direction
Software Program ProSort ™
Workstation Computer requirements 5 Quad Core; 8 GB RAM; 250 GB HDD, Windows 7 Pro x64
monitor Recommended 24” LCD; 1920 x 1080 resolution
Physical Dimensions (W x D x H) 70 x 65 x 65 cm deep (instrument only)
requirements Weight 90 kg (instrument only)
Electrical 120–230 VAC; 50–60 Hz
Temperature Ambient temperature range of 18–25°C
Noise <60 dBA while in operation
Options Biosafety cabinet S3 Biosafety System Class I
Regulatory Electrical Electrical Safety for Europe
status IEC 61010-1 CB Scheme
UL/CSA Standard for Safety Electrical Equipment for
Electrical Safety (USA, Canada, NRTL)
IEC61010-2-081, EN 61010-2-081
EN/IEC 60825-1, EMC EN 61326-1
CE Declaration of Certification
Laser Class I (1) laser product per CDRH regulations and EN/IEC 60825-1