Bio-Rad S3™ Cell Sorter - Manual (Page 19)

Instruction Manual | 9
2.3 Optics
The sort positions for each collection vessel are numbered and will correlate to the position
numbering in the software when the sort logic and limits are set. When placing tubes in the
sort chamber for sorting, it is recommended to add media or buffer to the tube to help prevent
sorted cells from drying out and to cushion their collection. The minimum recommended
volume is 0.5 ml of media or buffer for each 5 ml tube. Quick-attaching adaptors are available
for the 8-well strips or microscope slides. Adaptors are part of the accessory kit and they can
be kept inside the fluidics door for storage.
CAUTION! Biohazard! When running samples and sorting, hazardous aerosols may be
created depending on the sample type. To prevent hazardous aerosols from spreading,
keep the green containment door closed as often as possible. Consult with your local
safety officer or review local, state, and federal regulations to ensure proper handling and
disposal of biohazardous substances including samples, sorted fractions, and waste.
CAUTION! Shock hazard! The sort deflection plates in the sort chamber are
charged while sorting. An interlock on the sort chamber door will disable the plates
when opened. Only qualified personnel should override this interlock.
2.3 Optics
The S3 and S3e optics include the laser(s), mirrors, filters, and lenses, which guide the laser
light to the stream of sample and collect scattered and emitted light for detection.
CAUTION! Laser hazard! Laser radiation can be hazardous. Please do not override
optical interlocks or remove light shields, as they are in place for your safety. Only
trained personnel should access the exposed laser beam.
2.3.1 Laser
The S3 and S3e Cell Sorters are installed with a 488 nm, 100 mW laser for cell and particle
interrogation that passes through the system in the sample core. The laser power and shutter
can be controlled through the software interface. An optional 561 nm or 640 nm 100 mW laser
are available as a second excitation source.
Fig. 9. Sort collection chamber and deflection plates.
Deflection plates