Bio-Rad S3™ Cell Sorter - Manual (Page 47)

Instruction Manual | 37
4.4 Quality Control
Note: If the bead lot is not available in the dropdown menu, refer to Section 3.3.1, Editing
QC Criteria.
A series of windows will open during the QC procedure to perform alignment and calculate
drop delay settings. User interaction is not required for these windows. These steps are
described later in this section.
7. After completion, the Drop Delay Alignment Status window will open with the optimal drop
delay settings (Figure 36A).
8. Click the checkmark to open the QC Report.
9. Review the QC report (Figure 36B).
n
If the QC status passed, exit the window. The system is now ready for sample acquisition
and sorting.
n
If the QC status failed, rerun the QC procedure up to two more times.
4.4.1 Droplet Setup
The first step in the QC process is droplet setup. Droplet setup is performed automatically and
the droplets will be optimized to a stable break-off. The droplet camera image may be viewed
by clicking Droplet Monitor on the Home toolbar. The Droplet Monitor window will display the
drop delay, drop drive amplitude, and status of the drop delay (Figure 37).
Users with administrator privileges may access and modify these settings in the administrator
tab. For more information, refer to Section 3.3, Administrator Tab Toolbar.
Fig. 36. A, Drop Delay Alignment Status window and B, Daily QC Report.
A
B