Bio-Rad S3™ Cell Sorter - Manual (Page 109)

Instruction Manual | 99
10.4 Swap Nozzle Tip Wizard
20. Remove the lint-free cloth from nozzle chamber.
21. Press the nozzle stage back down. The nozzle stage should snap back into position
(Figure 117).
22. Click the blue forward arrow.
Fig. 117. AutoGimbal Swap Tip window.
23. Slide the sort chamber door open.
24. Clean the sort chamber to ensure there are no stray droplets on the back wall, sort tray, or
deflection plates, and that the streams camera window is clean (Figure 118).
25. Check to see if the stream is hitting the waste hole. If it is, proceed to step 39. If the stream is
hitting the back wall, sort tray, or deflection plates, remove and reseat the nozzle tip (Figure 119).
Note: The system will perform a series of debubble cycles before proceeding, which can be
heard by active clicking (Figure 120).