Bio-Rad S3™ Cell Sorter - Manual (Page 73)

Instruction Manual | 63
In front of each PMT is a holder for a neutral density filter or bandpass filter. Each filter holder
contains a screw-in retaining ring that can be removed or replaced with the provided red S3
spanner wrench.
5.4.2 Optical Filter Blocks
The S3 and S3e Systems include two preset filter blocks. The filter set depends on the
instrument configuration. The optical filter blocks can be changed to accommodate dectection
of different fluorophores. These blocks can be easily removed with the silver handle (Figure 69).
The filter blocks contain the dichroic and bandpass filters. The optical filters within the block
are user changeable and will accommodate multiple filter sizes as described in Table 23. The
dichroic filter holders are locked from the bottom of the block. Once unlocked, they can be
removed and replaced from the top of the block.
Table 23. Description of filter sizes for use with optical filter blocks.
Filter
Descripiton
Normal incidence filters 25.4 mm (1 in) round, up to 2 mm thick
12.7 mm (0.5 in) round, up to 2 mm thick
11 x 11 mm square, up to 2 mm thick
12 x 25.4 mm rectangle, up to 2mm
Dichroic holders 25.4 mm (1 in) round, up to 2.7 mm thick
12 x 25.4 mm rectangle, up to 2.7 mm thick
Neutral Density (ND) holders 25.4 mm (1 in) round, up to 10 mm thick
Fig. 69. Lifting an optical filter block.
5.4 Optical Filters