Bio-Rad S3™ Cell Sorter - Manual (Page 33)

Instruction Manual | 23
3.3 Administrator Tab Toolbar
Table 22. Administrator tab toolbar buttons and their functions.
Button
Name Function
Edit QC Criteria View and edit the CV and PMT voltage criteria for the quality control
procedure. This may be done as a result of a bead lot change
Edit Droplets Edit the settings for droplet creation. These settings include drop drive amplitude,
drop drive frequency, charge phase, defanning, and drop delay. These settings are
automatically adjusted as part of the QC procedure and will result in sort changes.
These settings are grayed out while the system is maintaining
Edit Streams View sort streams and edit the settings for stream creation. These settings
Settings include deflection, charge plate voltage, defanning, phase, and test pattern
adjustment
Print User Report View usage reports including times, dates, and duration of system use for
all users
Manage Users Create new users, reset passwords for existing users, set rights, and
delete users
Decontaminate Run a decontamination wizard that will walk user through a complete fluidic
system decontamination. This will take approximately 2–3 hr
Calibrate Sample Reset the differential offset sample pressures, which returns the preset
Pressure Offset buttons (low, med, high) to their correct ranges
Global preferences Change global instrument options for sheath concentration used, idle
shutdown time limit, and boost adjustment, plot default options and file save
default location
3.3.1 Editing QC Criteria
An administrator can edit the QC criteria used to pass or fail the instrument during the daily QC.
This should be performed only by an experienced user who has verified system performance.
The rewind arrow on the middle bottom of Edit QC Criteria window will reset the QC criteria to
the factory defaults for the system (Figure 18).
When receiving a new lot of ProLine calibration beads:
1. Download the bead lot file from the S3 or S3e Cell Sorter website,
www.bio-rad.com/cellsorter.
2. Click the green plus button (Figure 18).
3. Navigate to the new bead lot file.
4. Select the file and click Open.
The software will automatically update the QC criteria for the new lot of calibration beads.
Values in red indicate values that are not used for pass/fail criteria for QC. If you plan to enter
the bead lot information manually:
1. Verify the acceptance criteria on the certificate of analysis.
2. Compare the old lot to the new lot.
3. Enter the new lot information into the Edit QC Criteria window.
IMPORTANT! Changes to these criteria will apply globally to the system.