2 loading instrument settings, 3 saving protocols – Bio-Rad S3™ Cell Sorter User Manual

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Instruction

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4.5 Protocols and Workspace

4.5.2 Loading Instrument Settings

Protocols can be saved for future use. Each protocol is saved with the following information:
plots, regions, gates, trigger, threshold, PMT voltages, sort logic, sort limits, sort modes,
compensation settings, and filter configuration.

To load a previously saved instrument setting:
1. Open the protocol to be used or create a new protocol and save.
2. Click File > Load Instrument Settings.
3. Choose the protocol that contains the settings to be loaded.

The preexisting settings will be loaded into and displayed in the current protocol and will be
ready to use for acquisition or sorting.

4.5.3 Saving Protocols

Protocols can be saved for future use. Each protocol is saved with the following information:
plots, regions, gates, trigger, threshold, PMT voltages, sort logic, sort limits, sort modes,
compensation settings, and filter configuration.

To save a protocol (Figure 44), click File > Save Protocol.

Refer to Section 3.3.8, Global Preferences, for information on how to assign default folders for
saving both protocols and FCS files.

Fig. 44. File dropdown menu with New, Open, Load Instrument Settings,
and Save Protocol highlighted.

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