Bio-Rad S3™ Cell Sorter - Manual (Page 84)

74 | S3 and S3e Cell Sorters
Additional Software Features
7.4 Clean System
In between samples or users, cleaning can be done at low or high pressure. High pressure
cleaning will cause the system to stop maintaining droplets and a QC procedure must be run
before sorting. If cleaning between samples, the low pressure option is recommended. The
fluidics lines will be flushed thoroughly with cleaning solution, similarly to the cleaning protocol
used during the shutdown or logout procedure.
To clean the system:
1. Fill a 5 ml tube with a minimum of 3 ml of cleaning solution.
2. Load tube into the sample station and move in to run position.
3. Click the Set Up and Maintenance tab.
4. Click Clean System.
The cleaning protocol will automatically begin. A prompt will appear to warn the user that the
clean function will begin and that the contents within the sample tube will be used rapidly.
Fig. 78. Cleaning protocol warning box.
Fig. 79. Clean System modes.
Fig. 80. Warning for high pressure cleaning protocol.
5. Select the cleaning mode to run (Figure 79).
6. Click Yes to proceed with mode (Figure 80).
7. Move the loading stage into wash position (Figure 81).