Bio-Rad S3™ Cell Sorter - Manual (Page 29)

Instruction Manual | 19
3.1 Main Software Window
Table 17. Fluidics buttons and their functions.
Button
Name Function
Debubble Removes bubbles from nozzle
Unclog Allows user to pull vacuum in the case of a nozzle clog. Use this option before
removing the tip for sonication or replacing with a new nozzle tip
Swap Tip Stops the sheath flow to allow nozzle tip replacement. A wizard will appear to
walk the user through a nozzle change. Once the tip has been replaced, QC
needs to be rerun to verify alignment and set the drop delay
Swap Fluidics Allows fluidic containers to be swapped without shutting the system down.
Prepare a full replacement or empty container prior to pressing this button, as
a three-minute timer starts after the button is pressed
Clean Cleaning can be done at low or high pressure. High pressure cleaning will cause
the system to stop maintaining droplets and a QC procedure must be run before
sorting. If cleaning between samples, the low pressure option is recommended
Table 18. Other buttons and their functions.
Button
Name Function
Annotation Adds an annotation window to the workspace. Annotations added prior to
saving will be saved to the FCS file’s embedded protocol
Image Allows an image to be added to the workspace. The image can be moved
or deleted. If added prior to saving, this image will be saved to the FCS file’s
embedded protocol
Basic Adds basic information to the print page in the form of a header. This information
Information includes date, time, user login, serial number, filename, event source. If added
prior to saving, this information will be saved to the FCS file’s embedded protocol
Filter Configuration Adds the optical layout to the print page showing PMT and optical filter setup.
This can be modified to match the actual filters if they have been changed. If
added prior to saving, this will be saved to the FCS file’s embedded protocol
Table 19. Publish and user buttons and their functions.
Button
Name Function
Preview Displays a preview of the page layout(s) setup prior to printing
Page Orientation Changes between a printable landscape or portrait workspace
Print Opens the print dialog to select printer, number of copies, etc. and print.
Note: Use the print preview option to see where page breaks occur
Print Options Adds additional windows or instrument settings to the page layout for printing.
Change Password Allows the currently logged-in user to change password
System Log Opens window for system log. Displays date, time, error, error details. This
option is helpful when working with technical support and service engineers
for troubleshooting