Bio-Rad S3™ Cell Sorter - Manual (Page 122)

112 | S3 and S3e Cell Sorters
Troubleshooting
Table 30. Troubleshooting table (continued).
Error
Possible Causes Troubleshooting Steps
Drastic light change Background movement in Redo QC and avoid changing light
front of green sliding door conditions in front of green sliding door
during QC process during the procedure
Sheath error The internal sheath The connections to the DI water and 8x
reservoir cannot maintain sheath fluid containers should be checked to
proper levels make sure they are connected, that they
are not empty, and that fluid can flow. It is
possible that their filters are clogged and
need to be replaced
Waste error Waste quick disconnect The connections to the waste container
not connected properly to should be checked to make sure they are
waste container connected and that waste can flow. Once
corrected, it will take time for the system to
Internal waste reservoir filled
clear the internal reservoir. You can observe
that pumping is happening by watching for
splashing in the waste container
Nozzle stage cannot return to Laser intersection cover has Adjust laser intersection cover so it is seated
proper position after nozzle been moved during tip swap correctly. Once in the proper place the
tip swap nozzle stage can be lowered into position
Cannot acquire sample. Room too cold or faulty Warm room to >63°F. If this does not help,
Hovering on acquisition button bubble detector contact service support
displays “bubble detected”
Arcing The high voltage deflection Stream spray or droplets are on the
plate monitor has measured deflection plates. Carefully clean and dry
the plate voltage deviating the plates
from normal
No data appear on the work- Nozzle interlock has been Open nozzle door all the way then close to
space after changing nozzle tip activated reset the laser interlock
No events during acquisition Nozzle door open Close the nozzle door
Laser shutter closed Cycle the nozzle door open then closed,
especially if cover was removed
Laser not on Turn on the laser in the user interface
Restart instrument and computer to correct
bad communication
Threshold too high Lower threshold
Triggering on noise Change the trigger parameter to FSC or SSC
Stream not centered in Use the electronic micromotors (Agillis)
laser beam positioner while acquiring to maximize event
signal — access within the system log file
No beads in sample Check sample tube, vortex
continues