Bio-Rad S3™ Cell Sorter - Manual (Page 116)

Maintenance
106 | S3 and S3e Cell Sorters
A decontamination wizard will start and walk through each procedure. When prompted follow
the instructions.
3. Choose whether the system should be shut down after decontamination has completed
(Figure 129).
4. Click the checkmark button to proceed.
5. Select whether or not an automatic startup should be scheduled (Figure 129).
Fig. 128. Decontamination warning dialog box.
It is recommended to run the decontaminate procedure at least once every six months but can
be run as often as monthly. This tool should also be used if there is a noticeably high background
level in the acquired data. The source could be within the fluidic path. Bacteria or fungus can
potentially grow in the lines if samples are not handled using basic cellular sterile technique. The
bulk fluidics can also contribute to contamination, despite having internal filters built into the lines.
To run the Decontaminate tool:
1. Move the loading stage into the wash position.
2. On the Administrator tab select Decontaminate.
A warning window will appear to confirm that a decontamination procedure should be run.
Fig. 129. Automatic shutdown and automatic startup option in the decontamination procedure.
6. If yes, assign date and time for automatic startup procedure to run.
7. Click the checkmark button to proceed.