Bio-Rad S3™ Cell Sorter - Manual (Page 88)

Additional Software Features
78 | S3 and S3e Cell Sorters
IMPORTANT! The S3 Biosafety System must remain on at all times when the S3 or
S3e Cell Sorter is inside the system. The cell sorter should be left with the main
system power on and will enter into idle mode when not in use.
Should the S3 Biosafety System be turned off when the S3 or S3e Cell Sorter power
is on inside, there could be risk of overheating and it may become a fire hazard.
If any of the following occur, the S3 and S3e Cell Sorters will stop sample acquisition or sort
and alert the user:
n
Airflow through HEPA filter drops below required limit
n
Filter life reaches 0%
n
ProSort Software loses communication with the enclosure
Until resolved, the software will not allow running samples or sorting.
When performing the swap tip procedure, the front panel may be lifted and attached via magnet
to the upper filter housing to allow nozzle access. Note that in this state, the S3 Biosafety
System’s airflow is reduced and the system is not considered to be in Class I containment.
IMPORTANT! If the airflow through the HEPA filter falls below required limits
for containment, the software will alert the user and stop the sort or
acquisition immediately.
7.10 Biosafety System
The S3 ™ Biosafety System is a Class I aerosol containment hood designed to work with the
S3 and S3e Cell Sorter, which works in conjunction with ProSort ™ Software, providing users
and the environment protection from aerosols created during the cell sorting process.
The S3 Biosafety System is fully integrated with and monitored by the S3 and S3e Cell Sorter’s
ProSort Software, so users have real-time information about HEPA filter life and the temperature
of the system inside. To maintain containment, the fan speed is regulated based on the cell
sorter operation mode and the airflow rate through the HEPA filter.
The S3 Biosafety System functionally meets airflow requirements from the National Institutes of
Health (NIH) standards for Class I biosafety and the International Society for Advancement of
Cytometry (ISAC) guidelines.
For more information please see the S3 Biosafety System Class I manual.
Fig. 87. S3 Biosafety System Class I status window.