Bio-Rad S3™ Cell Sorter - Manual (Page 63)

Instruction Manual | 53
5.1 Aquisition Setup
5.1.2 Event Limit
An event limit may be set on the acquisition (Figure 55). This limit will be based on the total
events collected. The gate default is All. When this event limit is reached, the acquisition will
stop and the FCS file can be saved. The maximum number of events acquired in a single FCS
file is 100,000,000.
Note: Large FCS files may take time to save, load, and display.
A gate limit can be used to define the event limit. Each gate created on the plots will be listed on
the dropdown menu. Once a gate is chosen, the acquisition will continue until the event limit is
reached within the gate.
5.1.3 Cycle Mode
At the beginning of an acquisition, it may be helpful to utilize the cycle mode for a quicker
refresh of the data displayed while changing the settings (Figure 55). Cycle mode is set in units
of seconds and will progressively display events that fall within that period of time. Older data
will be not be displayed.
Use this function when adjusting PMT voltages and/or threshold and trigger settings. After data
and populations of interest appear where desired on your plots, disable this mode and restart
acquisition for normal cumulative acquisition.
5.1.4 Trigger
The trigger parameter is the initial parameter of detection to signal the system that a particle
of interest is present. Combined with the threshold setting, the trigger parameter defines real
events which should be detected and analyzed (Figure 56). Typically, a scatter parameter
is selected for the trigger, as it will identify all particles above a given size regardless of the
fluorescent signal, though any parameter can be used for this function.
Fig. 56. Target event rate and threshold level.
Fig. 55. Event limit and cycle mode.