Bio-Rad S3™ Cell Sorter - Manual (Page 99)

Instruction Manual | 89
10.4 Swap Nozzle Tip Wizard
10.4.1 Nozzle Tip Wizard with the Agilis System
The Agilis system uses two axis-motorized positioners that are controlled by the software.
These piezo-driven micromotors help align the stream to the pinhole and to the collinear lasers.
In addition, there are three gimbaling screws that are used to make manual adjustments to the
nozzle stage for nozzle height in relation to the pinhole and to the streams laser.
The nozzle tip must still be removed, cleaned, and reseated manually prior to alignment. The
stream must be hitting the waste hole to ensure successful results.
To swap the nozzle tip:
1. On the Setup and Maintenance tab select Swap Tip. The Swap Nozzle Tip wizard will open
(Figure 99). Follow the instructions indicated by the wizard.
2. Open the nozzle chamber door.
3. Press on the left side of the black metal light protection device and lift out of the way.
4. Locate the thumb screw hinge.
5. Loosen the thumbscrew until the nozzle body rises up and the tip can be seen.
6. Click the blue arrow.
Table 29. Cell sorter and nozzle systems.
Catalog #
Cell Sorter Nozzle System Proceed to Section
145-1001, 145-1002 S3 Cell Sorter Agilis 10.4.1
145-1001, 145-1002 S3 Cell Sorter (upgraded
with AutoGimbal ™ feature)
AutoGimbal 10.4.2
145-1005, 145-1006, 145-1008 S3e Cell Sorter AutoGimbal 10.4.2
10.4 Swap Nozzle Tip Wizard
The Swap Tip wizard will walk through each step necessary to perform a successful nozzle tip
swap. Read through the wizard windows carefully and follow instructions on each screen as you
proceed with the protocol.
Clear an area around the instrument to have adequate working space. At times, adjustment to
the nozzle stage is necessary. Handle the nozzle with clean gloves.
First, determine which nozzle system is installed in your cell sorter (Table 29).
Fig. 99. Nozzle swap wizard window.