Maintenance, 1 general maintenance, 1 daily – Bio-Rad S3™ Cell Sorter User Manual

Page 97: 2 weekly, 3 yearly

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Instruction

Manual | 87

Maintenance

Always follow the PPE guidelines relevant to your laboratory’s safety procedures for dealing
with the chemicals recommended below.

10.1 General Maintenance

10.1.1 Daily

It is recommended that the sample line is cleaned every day prior to shutdown. See Section
10.6, Disinfectants
, for details regarding approved cleaners for this tubing.

The system must be shut down through the software on a daily basis. If the system is not shut
down properly, it may be prone to software-instrument communication problems, and the
sample lines, nozzle, and fluidics may be prone to contamination.

If using the automated startup option, be sure to clear the waste and fill the fluidics at the end
of use to ensure no errors will occur.

10.1.2 Weekly

Each week, wipe down the system with a mild disinfectant. Clean any salt crystal buildup in
the sort chamber or on the deflection plates.

Inspect the camera windows for any debris or salt. Clean them with a swab and mild
disinfectant as needed.

10.1.3 Yearly

It is recommended to purchase the annual preventative maintenance (PM) plan offered with
the S3

and S3e

Cell Sorter. The PM includes but is not limited to an annual onsite visit by a

service engineer to:

n

Replace the inline sheath filter

n

Replace the inline DI filter

n

Replace the bulk fluid filters in the
sheath and DI bottles

n

Replace the air pump filter

n

Replace the inline filters

n

Replace the rinse filters

n

Clean the optical filters

n

Replace the nozzle assembly

n

Examine tubing and connectors

n

Perform system check

n

Verify and adjust optical alignment

10

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