Bio-Rad S3™ Cell Sorter - Manual (Page 17)

Instruction Manual | 7
2.2 Fluidics System
During washing, a new sample can be loaded into the sample input station. The status of
the loading stage is displayed by a locked or unlocked padlock symbol located on the touch
locking system screen (Figure 7).
To move the loading stage from the run position to the wash position:
1. Press the touch locking system screen until the padlock displays as unlocked. This will
depressurize and unlock the sample station.
2. Push the loading stage handle down, pull forward, and lift up.
WARNING! The loading stage uses a spring to push up into the run or wash position. To prevent
the loading stage from over-springing into position and possibly damaging the instrument,
guide the loading stage into the run or wash position by holding onto the handle for a smooth
movement into position.
Note: If the loading stage is in the wash position and another wash is required, press the touch
locking system screen to perform washing again.
The temperature of the sample input station and the sort tube holder can be controlled from the
ProSort Software via a single Peltier solid state system. The temperature range can be set from
4–37ºC in 1ºC intervals.
The sample input station is capable of mixing the sample. Sample mixing is performed by
vortexing. Users can set the vortexing speed to high, low, or off, depending on the sample type.
Note: It is recommended that resuspended adherent samples are mixed at the high speed to
prevent clumping.
IMPORTANT! It is highly recommended that samples are filtered prior to running. This
will minimize clogs in the sample tube and nozzle tip.
Fig. 7. Touch locking system screen.