Bio-Rad S3™ Cell Sorter - Manual (Page 77)

Instruction Manual | 67
6.1.1 Sort Collection
The S3 ™ and S3e ™ Systems offer four types of sort collection vessel options:
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5 ml tubes
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1.5 ml tubes
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8-well strips
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Microscope glass slides
Each sort direction has the capability of sorting into 5 ml tubes and 1.5 ml tubes up to a quantity
of 5 tubes in each direction. Using the quick-attaching adaptors, sorted cells can be collected
into 8-well strips (up to a quantity of 8) or onto a microscope slide (up to a quantity of 20). Each
tube and well may have different sort modes and limits assigned.
It is highly recommended that media, buffer, or serum is added to the sort tubes or wells prior
to sorting to give the cells a cushion, avoid evaporation of droplet volumes, and to help preserve
cell viability during the sort collection period. The minimum recommended volume is 0.5 ml of
filtered buffer or media for each 5 ml tube. The minimum starting volume for 1.5 ml tube is 0.1 ml.
Note: The sort output may be temperature controlled to the same temperature as the sample input
station. This temperature can be enabled/disabled and adjusted on the instrument control panel.
6.1.2 Volume Tracker
During a sort, the system has the ability to track the volume and number of droplets deflected
into each sample collection tube. Volume tracking can be enabled to automatically stop a sort
or move to the next tube position before a tube overflows.
To enable this feature:
1. Click the Prevent Overflow checkbox in the Sort Logic window (Figure 72).
2. Enter the starting volumes for the sort collection tubes.
Note: The minimum starting volume for a 5 ml tube is 0.5 ml and the maximum is 2 ml. The
minimum starting volume for 1.5 ml tube is 0.1 ml and the maximum is 0.5 ml.
Fig. 72. Sort Logic window with Prevent Overflow highlighted.
6.1 Sort Setup