Bio-Rad S3™ Cell Sorter - Manual (Page 43)

Instruction Manual | 33
Getting Started
4
After system installation by a Bio-Rad certified service engineer, the S3 ™ or S3e ™ System should
not be turned off via the main power switch. The system is safe in standby mode after performing
a software shutdown of the system. This will help facilitate quick or automated startup.
If the system is shut off via the main power switch, always power the instrument on before
starting the ProSort
™ Software. The internal computer system of the instrument must be
running in order to communicate with the software.
IMPORTANT! Powering the system on or off incorrectly can cause the instrument to
not run properly.
4.1 Checking Bulk Fluidics
Prior to starting the ProSort Software, open the bulk fluidics door and check the volume
of each container. Ensure that the waste container is empty, and the sheath fluid and DI
containers are full.
Note: To change the bulk fluidics after starting the software or during a run, refer to Section 5.3,
Checking or Swapping Fluidics.
To refill or change a container (Figure 29):
1. Detach the quick disconnect from the cap assembly by pushing in the metal button and
lifting up the quick disconnect until it engages the magnetic holder.
2. Slightly lift and pull out the container.
3. Remove the cap assembly from the container.
4. Refill or change the container.
5. Carefully place the cap assembly onto the container and tighten the cap.
6. Place the container into position.
7. Attach the quick disconnect to the cap assembly. An audible click should be heard.