Bio-Rad S3™ Cell Sorter - Manual (Page 104)

94 | S3 and S3e Cell Sorters
Maintenance
The window will give an option of repeating the gimball adjustment process or skipping and
proceeding to droplet calibration. If the camera views show that the stream is centered and the
nozzle line is green, droplet calibration can be done.
27. Close nozzle door.
28. Perform droplet calibration and QC to complete the tip swap process (Figure 109–112).
Prior to droplet calibration, the Adjust Stream Position window appear as a final check for
positioning of the stream (Figure 109). The pinhole image can also be accessed from this
window (Figure 110).
Figure 108 displays the stream image (left, center) and the nozzle camera image (right). The
bottom of the nozzle will be displayed as a double dashed green line.
26. Lower or raise the nozzle, using the three gimballs, until the orange line and the yellow line
meet and turn green.
The S3 Cell Sorter will again use the micromotors to align the stream to the pinhole and the
lasers. The three camera views will be shown again. The micromotors will bring the stream into
focus again. This second alignment process should complete the alignment.
Fig. 108. Nozzle swap wizard window.
Fig. 109. Adjust Stream Position window streams view.