Bio-Rad S3™ Cell Sorter - Manual (Page 71)

Instruction Manual | 61
3. Open fluidics door.
4. Detach the quick disconnect from the cap assembly by pushing the metal button.
5. Lift the quick disconnect until it engages the magnetic holder. This will hold the quick
disconnect away from exchange area.
For sheath fluid or DI water:
6. Slightly lift and pull out the empty container.
7. Remove the cap assembly from the empty container.
8. Uncap and remove the seal from a new 4 L container.
9. Carefully place the cap assembly onto the new 4 L container and tighten the cap.
10. Proceed to step 11.
For Waste:
6. Slightly lift and pull out the waste container.
7. Remove the waste cap assembly.
8. Empty the waste container following lab procedures for liquid biohazardous and chemical
waste removal.
9. Place the waste cap assembly into a new designated waste container or recap emptied
waste container.
10. Proceed to step 11.
To perform a hot swap:
1. On the Setup and Maintenance tab select
Swap Fluidics.
2. A countdown timer will automatically start. This is the amount of time left to swap the
containers before the system will automatically shut down (Figure 67).
WARNING! The system contains a limited amount of reserve fluids to continue running the
system during the swap. If the timer reaches zero, the system will automatically shut down.
Work quickly and carefully during these swapping steps. If the system shuts down, perform the
QC procedure prior to running a sample.
Fig. 67. Swap Fluidics window with swap time remaining highlighted.
5.3 Checking or Swapping Fluidics