KERN & SOHN AET 200-4NM - Manual (Page 95)

AET_/PET_/ILT_NM-BA-e-1410 95
23. PIPETTE CALIBRATION
Working mode < Pipette calibration> is dedicated to determining
measurement errors of volume in piston pipettes, in accordance with standard
ISO 8655 or according to user requirements.
In case of calibration carried out in accordance with standard ISO 8655, the
errors are automatically selected to tested volume, as specified in the standard
(see table of errors for standard ISO 8655).
Working mode enables:
• Calibrating pipettes with fixed or adjustable volume, single or multi-
channel;
• Preparing a database of pipettes including: pipette name, code, tested
volume and others;
• Calculating results on basis of:
Average volume of a pipette (channel)
Systematic error es (accuracy error)
Random error CV (repeatability error)
• Carrying out automated measuring procedures with consideration of
pipette’s type;
• Storing calibration results in a database (in a form of reports from
calibration process);
• Printing reports from pipette calibration process
• Exporting reports from tests
During volume testing procedure, the software determines accuracy and
repeatability errors for tested volume.
In case of pipettes with adjustable volume, the software enables declaring up to
5 volume from total pipette’s range, that are checked during calibration process.
In order to ensure the highest accuracy of pipettes calibration process,
maintain the following ambient conditions at a workstation:
 Ambient temperature of a pipette, tips and liquid should be kept between
20°C - 25°C with change rate during testing within ± 0.5°C
 Relative humidity 50 - 75%
and
 Use distilled water for pipettes calibration processes
 A pipette, tips and distilled water should be stabilized for temperature in
the weighing room. The reference norm advises that minimum
acclimatization time for above mentioned is 2 hours.
While calibrating process, balance user refers to the database of pipettes
including data on pipettes, its parameters, tested volume, and values of errors
determined for specific volumes.
Before carrying out calibration process, load database with data on pipettes and
calibration criteria.