Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 101)

• Replicate Count - Defines a quantity of replicate sets from one to nine.
• Grouping - Selects the sequence in which the replicates are laid out in plate wells. The
options are:
• 123123123 - This displays one of each replicate set at a time in numerical order.
• 111222333 - This displays all the replicates in a set before moving on to the next set in
numerical order.
You can assign the following well commands. Each command is associated with a color. You
can click and drag to highlight a series of wells, click a column or row header to highlight the
entire column or row, or simply click and highlight different wells and then click a command
below to assign that command to all the highlighted wells.
• Unknown (U): Yellow
• Background (B): Purple
• Control (C): Red
• Standard (S): Green
The Delete and Start at Well commands are also available to assign as well commands.
Delete removes the well command for the selected well. The Start at Well command enables
you to begin acquisition at a well other than A1.
NOTE: You should first delete all standards from the plate layout if any of
the standards need to be rearranged, and all controls from the plate
layout if any of the controls need to be rearranged.
NOTE: Wells and routine names you assign to the protocol plate layout are
saved into the protocol settings. The routine itself must exist on the
instrument. Standards and controls associated with a given protocol
typically remain constant, while the number of unknown wells often
vary. You can assign a specific number of unknown wells to the plate
when setting up a batch.
Commands and Routines - Assigns maintenance commands and routines to a well after
you select it in the Command Sequence area.
• Add - Opens the Commands and Routines dialog box so you can select a command or
routine.
•
• Delete - Deletes the selected command or routine.
• Post Batch Routine - Opens the Commands and Routines dialog box so you can select
a post-batch command or routine.
Protocols Page
87