Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 70)

changed to visually inspect the effect. Only the visual display of the dot plot and histogram
is affected for saved batches, not the acquired data.
NOTE: Gate positions are dependant upon buffer composition. Any changes
made to you assay buffer composition may result in a different
optimal gate location.
Right click inside the histogram area to access the following options:
X Axis - Use this function to change the X axis to the following: Doublet Discriminator,
Reporter 1, Classification 1, Classification 2, and Classification 3. Doublet Discriminator
is the default.
NOTE: You can change the X axis of the histogram for troubleshooting
purposes. Use only the default settings in all other instances.
• Gate - Show, create, delete, or move the gate. You can also move the gates by clicking
and dragging the red lines on the histogram.
• Autoscale - Click to automatically adjust the maximum number of events shown on the
Y axis. Click during acquisition to readjust the Y axis scale.
• Set Scale - The Set Scale dialog box displays. Set the maximum number of events and
click OK. The default number of events is 60.
• View- You can choose to view all events or only gated events.
• Display Mode - Opens the Histogram Options dialog box. Click either Logarithmic or
Linear display. Logarithmic display is the default. Select the Zoom check box for a
magnified view of the histogram.
• Dot Plot - The default location of the dot plot is the lower-right section of the Current
Batch tab. The dot plot is a graphical display of real-time data collection. Luminex
recommends using the default settlings to collect data.
Right-click inside the dot plot area to access the following options:
• X Axis - Changes the X axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, Classification 2, and Classification 3.
NOTE: You can change the X axis of the dot plot for troubleshooting
purposes. Use only the default settings in all other instances.
• Y Axis - Changes the Y axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, Classification 2, and Classification 3.
• View - Choose to view all events or only gated events.
• Display Mode - Select either Logarithmic or Linear display. The software defaults to
Logarithmic display. Click maximize for a magnified view of the dot plot.
• Plot Type - Select either Density Plot or Decaying Plot. The software defaults to
Density Plot, which displays a constant accumulation of events. Contrasting colors
indicate increasing density. The Decaying Plot plots only the last 100 events.
NOTE: The Density Plot allows the visual elimination of data values that are
determined to be insignificant to the display. Luminex recommends
collecting data in Density Plot mode to observe all collected events.
You can only view the Decaying Plot during batch acquisition.
Right-click inside the dot plot area to access the following options:
• Log - Displays a log of system processes. The log includes the following information:
xPONENT ® for FLEXMAP 3D ®
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