Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 104)

sets that are not in the sample. Therefore, choose only the bead sets present in your
sample.
• Apply All - Applies the information in the Units and Counts boxes to all analytes.
• Total Count - Select Stop after bead count reaches: to stop acquisition when the bead
count reaches a certain number determined by the user. Type the desired value in the box.
The default value is 50. You can also specify the minimum allowable bead count per well
that the xPONENT
® software analyzes. This excludes data from any beads carried over
during acquisition.
• Selected Analytes list - Selected analytes are displayed in a list on the right side of the
analyte grid. This list includes the following information:
• Name - The name of the analyte. Click and type to rename the analyte.
• Analysis - To change the type of analysis for an analyte, click this field to open the
Analysis Settings dialog box and select another analysis from the list.
In the Analysis Settings dialog box:
FIGURE 2. Analysis Settings Dialog Box
1. Select a method from the Method list.
2. If necessary, select a weight type from the Weight Type list.
3. Apply the analysis to all analytes in the list by clicking Apply to All Analytes.
4. Select Mark as Intra-Well Normalization Bead to make the analyte an intra-well
normalization bead. The normalization bead is a microsphere set that is included in
the assay as an internal control. It controls for sample variation and can be used to
normalize data between samples in a run.
5. Add a range to the analysis by clicking Add Range.
6. Select Use Threshold Ranges to enable ranges for the analysis.
7. Click Add Range to add a range.
8. Type a Range Name, a Low Value, a High Value, and select Inclusive if you want
to include the low and high values in the range. Click OK to exit the dialog box.
xPONENT ® for FLEXMAP 3D ®
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