Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 50)

5. Define the settings in the Acquisition Settings section. These are Volume, XY Heater
(enable/disable and set temperature), Plate Name, and enable/disable Sample Wash.
NOTE: Final washes are required for proper analysis. If you are not
performing a final wash step on your plate before acquisition on the
FLEXMAP 3D
® instrument, enable Sample Wash here. This
automatically washes each sample.
If you select Qualitative, Quantitative, or Allele Call from the Analysis Type list, you
can select Analyze results while acquiring samples to view real-time analysis.
6. Next, complete the Analysis Settings. These include Analysis Type, MFI enable/disable
(used for Allele Call analysis), # of standards, # of controls, either Fit all Standards or
Mean of Replicates.
7. Click Next. The Analytes tab opens. Click the analytes of interest in the numbered grid.
Information about the analytes opens in a list on the right side of the grid. Name the
analytes.
8. To change the Default Analysis, click Change. The Analysis Settings dialog box
opens.
9. Select the analysis method from the Method list. Click OK to change the default analysis
for analytes to be selected. Click Apply to All Analytes to apply the selection to all
analytes. The Analysis dialog box closes.
10. Type a unit of measurement in the Units box.
11. Type the desired bead count for each analyte in the Count box.
12. If you click Apply All, this applies to all analytes.
13. To change individual units or counts, change them in the analyte table.
14. Click Next. The Stds & Ctrls tab opens if you selected an analysis type other than None.
• If you are using an assay standard/control kit, click Apply Std/Ctrl Kit. In the Select
Std/Ctrl Kit dialog box, click the kit from the list and click OK. Applying a kit only works
for kits already installed, but you can also manually type the information.
• If you are not using a kit, type the appropriate information in the Standard Information
and Control Information sections. The number of standards and/or controls in these
sections is defined on the Settings tab in the Analysis Settings section. If your batch
uses controls, enter the appropriate values for Expected Values. Click Low Value
from the Show list, and enter the low value for each analyte. Click High Value from
the Show list, and enter the high value for each analyte. Reagent information is not
required for a custom batch unless you want to use the analysis feature.
15. Click Next. The Plate Layout tab opens.
• To add well commands, click the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• As you add commands to your plate, they are listed in the Command Sequence list.
Here you can give each of your wells an ID. You can also import an ID list and move
your commands up and down to change the plate location.
xPONENT ® for FLEXMAP 3D ®
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