Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 97)

add at least 25 µl to the sample well in addition to the sample size. The default value is 45
µl.
• XY heater - Select Enabled to enable the XY heater. In the box type the desired value in
degrees Celsius. The temperature range is 35° C to 60° C.
CAUTION: Acquiring data before the heater has reached the proper
temperature can compromise test results.
• Timeout - Check the Enabled box to enable the Timeout.
• Bead Type - Check the Bead Type box. From the drop down menu you can select
MicroPlex or MagPlex.
• Plate Name - Select the plate name from the drop down menu. Select Current 96-well
plate, Current 384-well plate, or a plate that you have created.
• DD Gating (Doublet Discriminator) - Use this to set the minimum and maximum DD Gating
values. In the first box, type the minimum value. In the second box, type the maximum
value. The default is 7500 to 15000.
• Reporter Gain - Select Standard PMT from the drop down menu to decrease the reporter
gain.
Analysis Settings - Use this section to set the analysis type, set the number of standards
and controls, select an external analysis program, and choose whether or not to analyze
results while acquiring samples.
• Analysis Type - Use this list to choose from the following analysis types:
• None - No analysis. Select if you have your own data post-processing program. You
cannot apply standards or controls when you select None. You cannot analyze
acquisitions with this setting.
•
• Qualitative - Qualitative analysis determines results as either positive or negative,
reactive or non-reactive. The software is flexible in defining custom result ranges, such
as negative, low positive, or high positive. Determinations are based on a single
standard. For qualitative analysis the Luminex software uses a specific algorithm, shown
below.
(FIsample)/(FIstandard) = Ki
Where FI = Fluorescent Intensity and Ki = a “Quali” value entered in lot information to
determine the value or the qualitative assay standard.
Two predefined formulas using the algorithm are included in the system. You can use
them as-is or edit their range values to meet your requirements.
• The “Quali” value determines a cutoff or threshold. This, in conjunction with ranges using
the Lum Qual formula or an edited range specific for your assay, helps to determine
qualitative results for unknown samples.
• Quantitative - Determines the sample concentrations from standard curves using
regression methods. Cubic Spline, Linear, Logistic 4P, and Logistic 5P. Type the desired
values for standards and controls in the Number of Standards and Number of
Controls boxes. Select either Fit of All Standards or Mean of Replicates for the
calculation of the curve fit.
Protocols Page
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