Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 59)

• 123123123. . . Lays out one of each replicate set at a time in numerical order.
• 111222333. . . Lays out all the replicates in a set before moving on to the next set in
numerical order.
You can assign the following well commands. Each command is associated with a color.
You can click and drag to highlight a series of wells, click a column or row header to
highlight the entire column or row, or click and highlight different wells and then click a
command below to assign that command to all the highlighted wells.
• Unknown (U): Yellow
• Background (B): Purple
• Control (C): Red
• Standard (S): Green
The Delete and Start at Well commands are also available to assign as well commands.
Delete removes the well command for the selected well. The Start at Well command enables
you to begin acquisition at a well other than A1.
NOTE: Before adding well commands, delete all standards from the plate
layout if any of the standards need to be rearranged. Delete all
controls from the plate layout if any of the controls need to be
rearranged.
NOTE: Wells and commands you assign to the protocol plate layout are
saved into the protocol settings and execute each time you use the
protocol to run a batch. Standards and controls associated with a
given protocol typically remain constant, while the number of
unknown wells often varies. You can assign a specific number of
unknown wells to the plate when setting up a batch.
Commands and Routines - Allows you to add and delete commands and routines, and to
create pre and post batch routines. Select a well and then select the appropriate command:
• Add
• Delete
• Pre Batch Routine
• Post Batch Routine
NOTE: If you select a routine you created, that routine must also exist on
any system to which you import this protocol. The system displays
an error when attempting to run a batch on a system where the
routine does not exist.
Clicking either Pre Batch Routine or Post Batch Routine opens the Commands and
Routines dialog box, where you can select the command or routine you want before or after
running the batch. Clicking Add after selecting a well opens the same box for you to select a
command or routine for that well. Clicking Delete after selecting a well deletes any
commands or routines associated with that well.
• Plate - Specifies the plate to display in the plate image in the list. Add Plate adds a new
plate to the batch, and Delete Plate deletes the plate highlighted in the list.
• Direction - Specifies the direction to run the plate commands. Select either horizontally or
vertically. The selected direction also dictates how wells are added to the plate when
assigning multiple unknowns, standards, and controls at one time.
Batches Page
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