Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 69)

• Analyte - Contains a list of analytes run in the batch. Select an analyte to view all statistics
for that analyte.
• Well(s) to View
• Current Well - Displays the statistics of the well currently being displayed. (This
changes to Displayed Well if viewing a batch using the Open button of the Saved
Batches tab.)
• Single Step - Instrument analyzes one well at a time. Click to turn function on or off.
This is useful to run prior to running an entire batch to confirm that the system is set up
correctly.
• Results pane - Displays statistics related to the batch. Use the up, down. left and right
arrow buttons to move through the table, or use the scroll bars. The maximize/minimize
toggle button expands the batch table and returns it to the standard size.
Plate - Select the plate you want to view, if there is more than one plate.
WARNING: If using multiple plates, ensure that plates are used in the proper
order. Failure to do so can result in inaccurate data and test
results.
Well Report pane - This pane displays a representation of the plate and the status of
acquired wells. Each well displays one of three possible states:
• Histogram/Dot Plot - Displays real-time monitoring of batch sampling during
acquisition.Both the histogram and the dot plot have buttons that enable you to change the
default view:
• Click the maximize button to maximize the graph to fill the whole window. In the
maximized view, use sliders to zoom in or zoom out. To return to the standard view, click
the minimize button. If the histogram or dot plot display have been changed to another
view (e.g. Progress, Well Report), click Default to return to the default histogram and
dot plot displays.
• The dot plot default display when using 1 to 100 bead regions Classification 1 and
Classification 2. When running regions greater than 100 beads, the default is
Classification 2 and Classification 3 axes, and radio buttons are displayed on the right
for the Classification 1 axis slices. To view the dot plot, you must use the default axis.
To display the bead set information, hover over the desired region. Click the upper view
classification button immediately below the maximize button to change the display to
show Classification 2 and Classification 3. This view displays a series of radio buttons
on the right side of the dot plot. When one of the radio buttons is clicked, the bead
regions in view are those in a fixed range of CL1 values plotted against CL2 and CL3.
Click the bottom view classification button to change back to the default classification
display.
• Histogram - The default location of the histogram is the lower left section of the Current
Batch tab. The Doublet Discriminator (DD) displays on the X axis. Events display on the
Y axis. Doublets appear when two beads stick together, creating undesired results. When
you select the gate, two vertical dashed red lines appear. These represent gate positions
determined by the protocol. Once the gate is set, everything outside of it is ignored. For
batches created using an existing protocol, the gate cannot be changed while running the
batch. For new batches created from new protocols, the gate can be changed while
running the batch. Any changes made to the gate while running new protocol batches
immediately affect the visual display of the dot plot and histogram. In addition, changes
made to the gate while running new protocol batches affect the acquisition for all wells
initiated after the change is made. When viewing saved batch data, the gate can be
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