Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 25)

NOTE: If using a strip-well plate, ensure that a strip is present in the
selected well location, prior to calibration.
5. Place the plate on the plate holder with A1 in the top left corner. Place the off-plate
reagent block on the plate carrier. Place a well-strip (provided with the Calibration and
the Performance Verification kits) in column S1 of the block.
NOTE: Make sure there is no liquid in the plate or the off plate reagent
block.
6. Click Retract to retract the plate carrier.
7. Type a name for the plate in the Plate Name box, or select a saved plate from the Plate
Name list.
NOTE: If a saved plate is selected the results of the new calibration will
override the previous calibration.
8. In the Plate section, click D6 for the 96-well plate. Click H12 for the 384-well plate.
9. In the Reservoir section, click RB1.
10. In the Strip-Wells section, click SD1.
11. Click Auto Adjust Height. The probe automatically adjusts itself and saves it to the plate
you selected.
NOTE: The probe height is automatically set to 0.49 mm. The probe
automatically adjusts this distance from the bottom of the plate or
calibration disks.
NOTE: When you adjust and save the probe height settings for all three
under a plate name, all areas retain the adjustment.
Introduction
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