Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 52)

CAUTION: Acquiring data before the heater has reached the proper
temperature can compromise test results.
• Timeout - Check the Enabled box to enable the Timeout.
• Bead Type - Check the Bead Type box. From the drop down menu you can select
MicroPlex or MagPlex.
• Plate Name - Select the plate name from the drop down menu. Select Current 96-well
plate, Current 384-well plate, or a plate that you have created.
• DD Gating (Doublet Discriminator) - Use this to set the minimum and maximum DD
Gating values. In the first box, type the minimum value. In the second box, type the
maximum value. The default is 7500 to 15000.
• Reporter Gain - Select Standard PMT from the drop down menu to decrease the
reporter gain.
• Analysis Settings - Displays the analysis type to be used for the batch.
Analysis Type - Use this list to choose from the following analysis types:
• None - No analysis. Select if you have your own data post-processing program and
want to obtain only fluorescent intensity results. You cannot apply standards or
controls when you select None. You cannot analyze acquisitions with this setting.
• Qualitative - Qualitative analysis determines results as either positive or negative,
reactive or non-reactive. The software is flexible in defining custom result ranges,
such as negative, low positive, or high positive. Determinations are based on a single
standard. For qualitative analysis the Luminex software uses a specific algorithm,
shown below.
(FIsample)/(FIstandard) = Ki
Where FI = Fluorescent Intensity and Ki = a “Quali” value entered in lot information to
determine the value or the qualitative assay standard.
• The “Quali” value determines a cutoff or threshold. This, in conjunction with ranges
using the Lum Qual formula or an edited range specific for your assay, helps to
determine qualitative results for unknown samples.
• Two predefined formulas using the algorithm are included in the system. You can use
them as is or edit their range values to meet your requirements.
• Quantitative - Determines the sample concentrations from standard curves using
regression methods Cubic Spline, Linear, Logistic 4P, and Logistic 5P, , and. Type
the desired values for standards and controls in the Number of Standards and
Number of Controls boxes. Select either Fit of All Standards or Mean of
Replicates for the calculation of the curve fit.
NOTE: Luminex ® recommends Fit of All Standards as the most accurate
calculation of the curve fit .
Based on a range of quantitative, numerical results, a threshold range can be applied
to a quantitative analysis, for example, high, low, saturated, and expected.
• Allele Call - Sets analysis for an allele call. Analytes must be put in groups of 2, 3, or
4 analytes.
• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call
analysis. Type a value in this box to set the minimum MFI for analysis.
xPONENT ® for FLEXMAP 3D ®
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