Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 112)

• Remove Air Bubbles (Luminex)
• Remove Clog (Luminex)
• Revive After Storage (Luminex)
• Verification (Luminex)
• Weekly Maintenance (Luminex)
Commands - This section contains the following commands:
• Wash - Sends distilled water through the fluidic lines in the system. It pulls the fluid from a
well or from the reservoir and runs it completely through the system to the waste
receptacle.
• Soak - Prevents salt crystals from forming in the probe due to air exposure. Soaking the
probe replaces sheath fluid in the probe with water. You should perform the soak function
at the end of each day. The system uses at least 250 µL of distilled water.
• F3DCAL1 - Calibrates DD, CL1, CL2, and CL3 for original non-magnetic xMap beads.
• F3DVER1 - Verifies DD, CL1, CL2, and CL3 for non-magnetic xMap beads.
• Alcohol Flush - Removes air bubbles from the sample tubing and the cuvette using 70%
isopropanol or 70% ethanol. The alcohol flush takes about five minutes. Alcohol Flush
uses the the off-plate reservoir because it can accommodate the amount of fluid necessary
to flush the instrument.
• Warm-up - Warms the system to prepare the optics prior to sample acquisition. The
system automatically begins warming up when you turn power on. The process takes 30
minutes. You will need to use the Warm-up command if the system is idle for four hours or
longer.
• F3DeCAL1 - Calibrates DD, CL1, CL2, and CL3 for original magnetic MagPlex beads.
• F3DeVER1 - Verifies DD, CL1, CL2, and CL3 for magnetic MagPlex beads.
• Sanitize - Uses the off plate reagent area, because only the reservoir can accommodate
the amount of fluid necessary to sanitize the instrument. The sanitize command performs a
similar function as the alcohol flush command, but uses 10% to 20% household bleach and
water solution to decontaminate sample lines and the cuvette after biohazard contact.
Sanitize as part of your daily shutdown routines.
• Prime - Removes air from the system's fluidic pathways by drawing sheath fluid from the
sheath fluid container. You do not need to supply solution in a plate.
• F3DCAL2 - Calibrates xMap ® reporters.
• F3DVER2 - Verifies RP1 at normal and high PMT and extended ranges for all xMap ® Bead
Types.
• Drain - Helps remove debris from the bottom of the cuvette during troubleshooting. You do
not need to supply solution. Draining takes approximately two minutes and should be
followed by an alcohol flush with 70% isopropanol or 70% ethanol.
• Backflush - Removes obstructions from the fluidics pathways by drawing sheath fluid from
the sheath fluid container.
WARNING: Do not run three backflush commands in a row. This will use the
sheath fluid faster than the system can replenish.
• F3DCAL3 - Calibrates extended RP1 ranges for all xMap beads.
• Fluidics - Fluidics calibrates sample carry over from well to well.
xPONENT ® for FLEXMAP 3D ®
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