Eppendorf epBlue with MultiCon - Manual (Page 56)

epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
56
6.3.1.6 Testing the changed liquid type
Prerequisites
• The parameters of the liquid types are customized to your requirements.
1. Carry out the sample run of the application with demineralized water.
2. Check the precision and accuracy of the dispensing.
3. Check the working volume of the labware. No liquid may spray out of the labware.
4. Carry out the sample run of the application using the provided liquid.
5. Check the precision and accuracy of the dispensing.
6. Check the working volume of the labware. No liquid may spray out of the labware.
6.3.2 Reagent transfer command
The Reagent transfer command is used to transfer a liquid from one or more source positions into several
destination positions. After the first source position has been emptied, the epMotion switches to the next
source position.
Abb. 6-12: Principle of the Reagent transfer command
Fig. 6-12: Principle of the Reagent transfer command
When using multiple source positions and the optical sensor is switched on (see p. 80), the volume of the
first source position will be checked when starting the application run. If there is not enough liquid
available for the number of samples, the
Checkrun window appears. To incorporate the next source position
in the calculation, select accept level and continue . The optical sensor checks the next source position. The
calculated volumes will be summed. The application will start if the volume is sufficient.
The Number of samples command is used to define the number of destination vessels.
The parameters of the command Reagent transfer are identical to the command Sample transfer (see p. 48).
Proceed as follows to define the pattern for the Reagent transfer command:
1. Select the source position in the source labware. If you are using several source positions, select these
vessels one after the other.
2. Select the destination position in the destination labware.
Source position and destination position for one pattern are the same color.