Eppendorf epBlue with MultiCon - Manual (Page 58)

epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
58
6.3.3.1 Example of a dilution series
This example explains the concept of a dilution series. The example does not describe the full application.
Objective
• 24 samples are in a rack with 24 positions and should be diluted 1:1000.
• The dilution should be completed in 3 program steps with dilutions of 1:10.
• First, a single-channel dispensing tool is used. An eight-channel dispensing tool will then be used to
reach a higher speed.
Procedure
• 200 µL of each of the samples will be transferred to the 96-well plate using the Sample transfer
command. The dilutions are carried out in this plate.
• 225 µL of the diluent will be transferred from the 300 mL reservoir to each of the empty wells of the
96-well plate using the Reagent transfer command.
• 25 µL of the sample will be aspirated from column 1 using the Dilute command and will be mixed with
respectively 225 µL of the diluent in the columns 2, 3 and 4. The mixing process will be repeated twice
in the following columns.
In this example, each dilution step is a 1:10 dilution. The required 1:1000 dilution will be achieved with the
third 1:10 dilution. The volume aspirated from the undiluted sample also applies for the dilution steps.
Creating dilution series
Transferring undiluted samples to the destination labware
1. Pattern of the Sample transfer command:
The source position and destination position are the same color. epBlue detects the pattern and
suggests the next field.